An analysis of epileptogenesis-related proteins involved in cAMP signaling

Dashtban Moghadam, Elahe; Khajeh, Khosro; Khodaverdian, Shima; Dabirmanesh, Bahareh; Mirnajafi-Zadeh, Javad; Fatholahi, Yaghoub

doi:10.22034/cmr.2024.7642.3086

An analysis of epileptogenesis-related proteins involved in cAMP signaling

Document Type : Research Paper

Authors

elahe dashtban moghadam ¹

khosro khajeh ¹

shima khodaverdian ¹

bahareh dabirmanesh ¹

Javad Mirnajafi-Zadeh ²

Yaghoub Fatholahi ²

¹ Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran

² Department of Medical Physiology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

10.22034/cmr.2024.7642.3086

Abstract

Epilepsy is a chronic neurological disorder that lacks an effective treatment, and most patients do not respond to existing medications. It is due to the lack of a molecular mechanism of epilepsy and the incomplete understanding of proteins that are involved that epilepsy is largely unknown. It is therefore important to identify and control the epileptic process so that relevant and effective drug treatments can be administered. This study used the electrical kindling method and the proteomics shotgun technique to analyze the proteomes of rats during epileptogenesis. From the analysis of system biology data, it was determined that the cAMP signaling pathway is involved in epilepsy, as well as changes in the expression of proteins that are upstream and downstream of this secondary messenger in the stages of electrical kindling was observed. There was a significant decrease in expression of somatostatin neuropeptide from upstream proteins and an increase in expression of phospholipase D2 and AMPA glutamate receptor type 2 from downstream proteins during epilepsy. Taking advantage of these data, we can consider a new method to identify the mechanism of epilepsy and introduce new drug targets by identifying changes in the expression of cAMP signaling pathway proteins and their roles in the epilepsy process.

Keywords

shotgun proteomics

epileptogenesis

electrical kindling

hippocampus

Subjects