Document Type : Research Paper

Authors

• Sepideh Sahebi
• Seyyedeh Mahdokht Maddah
• Golaleh Mostafavi

Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

Abstract

Lung and Gastric cancer are one of the most common types of cancer in the world and also in Iran. For finding therapeutic ways, researchers have been focused on changes in carcinoma cells at molecular level. Some natural phytochemical compounds affect apoptosis path and play an important role in producing and regulating processes involved in cancer. In the present research, the toxic effect of hydro-alcoholic extract of Nepeta glomerulosa on A549 and AGS cell lines, and p53 expression changes have been evaluated. The plant shoots were collected from Binalud region from Khorasan Province and 80% ethanolic extract was prepared by maceration technique. The cytotoxic properties of extract on two cell lines in three times, i.e., 24, 48, and 72 hours were assessed by MTT assay. Apoptosis induction amount was evaluated by DAPI staining. p53 gene expression alterations were determined using Real-time PCR. According to the results, the IC50 was 15 and 7.5 mg/mL, for A549 and AGS respectively (P<0.05). The results of DAPI staining showed that under the influence of the extract at IC50 concentrations, apoptosis was induced in both strains compared to the control. The increasing effect of p53 gene expression on AGS was more that of reported for A549 cell line (P<0.05) that demonstrates the more antiproliferative effects of this species on gastric cancer cells through increasing p53 gene expression and apoptosis induction.

Keywords

• apoptosis
• p53 gene
• cellular toxicity
• Nepeta glomerulosa

Main Subjects

• Cellular biology