Fabrication of Biosensor based on Polyaniline Nanostructure/Silver Nanoparticles for Stabilizing Glucose Oxidase Enzyme and Measuring Blood Glucose in Diabetic Patients

Document Type : Research Paper

Authors
Mahsa Mahdavinia 1
Gholamreza Kiani 2
Ayub Karimzad Ghavidel 3
1 Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
2 Department of Organic Chemistry and Biochemistry, University of Tabriz, Faculty of Chemistry, Tabriz, Iran
3 Department of Mechanical Engineering, Technical and Vocational University, Tehran, Iran
10.22034/cmr.2025.2298
Abstract
In the current research, a bio-sensor was fabricated to measure the blood glucose levels of diabetic patient using glucose oxidase enzyme. The produced bio-sensor is of the amperometric type, wherein polyaniline and silver nanoparticle were utilized as the enzyme stabilizer and sensitivity enhancer, respectively. The properties of the produced sample were investigated using electron scanning microscope and energy-dispersive X-ray spectroscopy. The morphological studies revealed that the nanocomposite layer of polyaniline/silver nanoparticles consistently covered the enzyme surface. During laboratory evaluations, an acceptable linearity was achieved within the concentration range of 60-160 mg/dl, with a sensitivity of 1.38 nA.dl/mg and a response time of 8 second. The bio-sensor output for real blood samples, within a temperature of 20-35 °C and glucose levels of 70-160 mg/dl, exhibited a significantly linear relationship with a sensitivity of 1.6 nA.dl/mg, representing a 14% improvement compared to previous samples. Statistical analysis indicated that the electrical current output of this sensor was independent of temperature and blood pH factors, thereby demonstrating that the designed biosensor can be successfully applied in clinical cases.

Keywords

Subjects

Cellular and Molecular Research (Iranian Journal of Biology)
Volume 38, Issue 2
Summer 2025
Pages 143-158

  • Receive Date 08 May 2023
  • Revise Date 19 July 2023
  • Accept Date 01 August 2023