Document Type : Research Paper

Authors

• Hasan Diba
• Jafar Hemmat
• Mohsen Vaez

Biotechnology Department, Iranian Research Organization for Science and Technology ‎‎(IROST), Tehran, Iran, ‎

Abstract

Thermostable Endo-1,4-β-D-glucanase, known as a density reducer in cellulose ‎degradation, is one of the most widely used enzymes in industries. In this study, the ‎thermostability of Endo-1, 4-β-D-glucanase produced by Bacillus sp. AGh1 which was isolated from ‎a hot-spring in Ardabil province, Iran was evaluated. The enzyme production was optimized through two steps. Rice bran and yeast extract ‎were determination as the effective factors for enzyme production by Plackett-‎Burman method, and optimization of culture medium was performed by Response Surface ‎Methodology method. The amount of mentioned effective factors for producing the enzyme ‎were 58.8 and 14.87 g L-1, respectively. The optimum temperature and pH ‎for the enzyme activity were 70 ̊C and pH 5.6, respectively. Accordingly, Bacillus sp. ‎AGh1 can produce endo-1,4-β-D-glucanase enzyme from rice bran as cheap raw ‎material with suitable activity. Consequently, the enzyme has functional at the wide ‎range of pH and may be applied which needs more studies.‎

Keywords

• Bacillus, Cellulase, thermostability, Endo-1,4-&beta
• -D-glucanase

Main Subjects

• Biotechnology