1
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2
Associate Professor, Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Introduction: L-asparaginase enzyme has various applications, particularly in medicine, pharmaceutical fields and food industries. The aim of this study was to optimization and characterization of free-glutaminase L-asparaginase activity.
Material and method: The primary isolation was performed on modified M9 medium. The colonies with pink color zone were selected for asparaginase and glutaminase activity study. Enzyme activity was determined using Nessler’s method. The effect of some factors such as incubation time, temperature, pH and metal ions on asparaginase activity were assayed.
Results: In the present study, 17 isolates were obtained that shown asparaginase production. One of them was selected as superior because of high asparaginase activity without L-glutaminase activity. The 16SrRNA sequencing showed that the isolated strain was similar 99.9% to Bacillus subtilis sbu-1. The asparaginase enzyme produced by this has the highest enzyme activity (8.80 U/ml) at pH 7، 40 oC. Also Co2+ and Ca2+ increase the asparaginase activity to 112% and 107%, respectively.
Discussion and conclusion: The obtained results in this study can help to introduce a new free-glutaminase L-asparaginase with reduction in toxicity, sensitivity and side effects of drug consumption.
gholami,N. , ebrahimipour,G. H. and yaghoubi avini,M. (2025). Isolation, identification and studies on biochemical characterization of extracellular free-glutaminase asparaginase isolated from the soil. Cellular and Molecular Research
(Iranian Journal of Biology), 38(2), 109-126. doi: 10.22034/cmr.2025.2294
MLA
gholami,N. , , ebrahimipour,G. H. , and yaghoubi avini,M. . "Isolation, identification and studies on biochemical characterization of extracellular free-glutaminase asparaginase isolated from the soil", Cellular and Molecular Research
(Iranian Journal of Biology), 38, 2, 2025, 109-126. doi: 10.22034/cmr.2025.2294
HARVARD
gholami N., ebrahimipour G. H., yaghoubi avini M. (2025). 'Isolation, identification and studies on biochemical characterization of extracellular free-glutaminase asparaginase isolated from the soil', Cellular and Molecular Research
(Iranian Journal of Biology), 38(2), pp. 109-126. doi: 10.22034/cmr.2025.2294
CHICAGO
N. gholami, G. H. ebrahimipour and M. yaghoubi avini, "Isolation, identification and studies on biochemical characterization of extracellular free-glutaminase asparaginase isolated from the soil," Cellular and Molecular Research
(Iranian Journal of Biology), 38 2 (2025): 109-126, doi: 10.22034/cmr.2025.2294
VANCOUVER
gholami N., ebrahimipour G. H., yaghoubi avini M. Isolation, identification and studies on biochemical characterization of extracellular free-glutaminase asparaginase isolated from the soil. Cell Mol Res, 2025; 38(2): 109-126. doi: 10.22034/cmr.2025.2294
Cellular and Molecular Research
(Iranian Journal of Biology)