Document Type : Research Paper

Authors

• nasrin gholami ¹
• gholam hosein ebrahimipour ²
• mohammad yaghoubi avini ¹

¹ Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

² Associate Professor, Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract

Introduction: L-asparaginase enzyme has various applications, particularly in medicine, pharmaceutical fields and food industries. The aim of this study was to optimization and characterization of free-glutaminase L-asparaginase activity.

Material and method: The primary isolation was performed on modified M9 medium. The colonies with pink color zone were selected for asparaginase and glutaminase activity study. Enzyme activity was determined using Nessler’s method. The effect of some factors such as incubation time, temperature, pH and metal ions on asparaginase activity were assayed.

Results: In the present study, 17 isolates were obtained that shown asparaginase production. One of them was selected as superior because of high asparaginase activity without L-glutaminase activity. The 16SrRNA sequencing showed that the isolated strain was similar 99.9% to Bacillus subtilis sbu-1. The asparaginase enzyme produced by this has the highest enzyme activity (8.80 U/ml) at pH 7، 40 oC. Also Co2+ and Ca2+ increase the asparaginase activity to 112% and 107%, respectively.

Discussion and conclusion: The obtained results in this study can help to introduce a new free-glutaminase L-asparaginase with reduction in toxicity, sensitivity and side effects of drug consumption.

Keywords

• L-asparaginase
• glutaminase activity
• Bacillus subtilis

Main Subjects

• Microbiology