Investigating the effect of prebiotic Biomin EPE on the total bacterial counts and Lactobacillus bacteria in the intestines of Sterlet (Acipenser ruthenus) and Bester cultured (Huso huso × Acipenser ruthenus)

Document Type : Research Paper

Authors

1 Department of Fisheries, Islamic Azad University, Bandar Anzali Branch, Bandar Anzali, I.R. of Iran

2 National Fish Processing Research Center, Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, I.R. of Iran.

3 Department of Fisheries, Islamic Azad University, Talesh Branch, PO Box: 43715-1161 Talesh, Guilan, I.R. of Iran.

10.22034/cmr.2024.2286

Abstract

Lactobacillus in the intestine of fish act as biological protector and can lead to the improvement of the health of meat after catching, therefore increasing their number in the intestine is considered important. The aims of this study were evaluating the effect of biomin on the total bacterial counts and Lactobacillus in the Bester and Sterlet intestine, their growth rate, and comparing them with the control. The treatments included fish fed with zero (control), 1, 1.5, and 2 g/kg biomin. 72 fish of each species with an average weight of 48 and 86 g based on 5% weight were fed by hand for 60 days. At the end of farming period, the length (44.50-30.95 cm) and weight (112.76 - 374.36g) showed a significant increase in the 2% Biomin compared to other treatments. With the increase in the amount of biomin in the diet, despite the decrease in total bacterial counts, Lactobacillus increased (P>0.05). Total bacterial counts between Sterlet (4.21-4.41CFU) and Bester (4.32–4.49CFU) in concentrations of 1-2% biomin compared to the control (4.49-4.58CFU) did not show a significant difference (P>0.05). At the same levels in Sterlet (1.46-1.89CFU) and Bester (1.49-1.94 CFU) compared to the control (1.39-1.45CFU) a higher of Lactobacillus was observed (P>0.05). Bacterial agents did not differ significantly between experimental treatments (P>0.05). Since the use of Biomin (2%) Lactobacillus increased in fish compared to the control and led to an increase in the growth of fish, therefore its use is recommended for feeding fish.

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Volume 37, Issue 1
March 2024
Pages 1-15
  • Receive Date: 22 December 2022
  • Revise Date: 22 May 2023
  • Accept Date: 10 June 2023