Document Type : Research Paper

Authors

• Bijan Bambai ¹
• Afagh Bapirzadeh ²
• mitra salehi ³
• zarrin minuchehr ⁴

¹ faculty member of national institute of genetic engineering and biotechnology

² researcher

³ Faculty of Islamic Azad University, North Tehran Branch, Department of Biological Sciences, Heravi Square, South Makran St., Postal Code 1651153311

⁴ Head of Nanotechnology Committee in National Institute of Genetic Engineering & Biotechnology.

Abstract

NSP1 protein is the first produced proteins of SARS-CoV-2, the virus which is responsible for Corona disease, sited at the beginning of 5́ end of virus gene number one. NSP1 bonds to Human 40S ribosomal subunits has potential roles in host cells mRNA translation inhibition.to clarify subtle molecular reasons of carrier animal’s pathogenicity, we used bioinformatics tools and 7k5i file structure acquired from PDB data bank to investigate interactions and internal bonds of 40S ribosomes. Majority of NSP1 interactions involve 18SrRNA ribosomal subunit and one of ribosomal proteins. These bonds are at A605and G600,601 nucleotide positions in 18SrRNA leading to both physically and spatially disrupting existing internal interactions in this subunit which eventually could cause ribosome to lose its function. Additional to 18SrRNA, NSP1 also bonds with three ribosomal proteins: S2, S3 and S30.alignment comparison of these molecules in Human and other mammalian in one hand and between Human and carrier animals such as camel and manis in another hand showed for example camel S2 protein is more like manis instead of being genetically close to human or cows. These crucial findings strongly support important role of NSP1 in translation inhibition of host cells

Keywords

• "
• SARS-CoV-2"
• NSP1"
• 40s ribosomal subunits"
• ، "
• Bats"
• ،"
• Manis"

Main Subjects

• Bioinformatics