Genomic and proteomic bioinformatics analysis of two heat shock proteins (HSP90-a and HSP90-b) of Tali goat breed and their comparison in seven species of domestic animals

Document Type : Research Paper

Author

department of biotechnology, graduate university of advanced technology

Abstract

Heat shock proteins (HSPs) play an important role in responding to various environmental stresses. In this study, genomic and proteomic characteristics of HSP90-a and HSP90-b in Tali goats in addition to 7 species of domestic animals including sheep (O. aries), goat (C. hircus), water buffalo (B. bubalis), cattle (B. taurus), Zebo cattle (B. indicus), one-humped camel (C. dromedaries) and two-humped camel (C. bactrianus) were analyzed using Multiple sequence alignment, SWISS modeling and phylogenetics analysis tools. The location of HSP90-a and HSP90-b gene is determined in all of studied species but its position has not been determined in the two humped camels. The number of exons for HSP90-a and HSP90-b in all studied species except two camel species were similar. Comparison of mutations in the Tali breed with the reference goat showed that the mutations of the exon regions have not caused any change in amino acids. Analysis of the Expasy translation tool also showed that HSP90-a and HSP90-b encode 733 and 724 amino acids for all species, respectively, except for the two-humped camel, which has 649 amino acids for the HSP90-a protein. The results showed that HSP90-a protein had one variable amino acid in water buffalo and six variable amino acids in two-humped camel, while only one-humped camel and two-humped camel had two variable amino acids in HSP90-b protein. This study will be supportive in selection of target genes for molecular adaptation of livestock.

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Articles in Press, Accepted Manuscript
Available Online from 30 November 2022
  • Receive Date: 26 December 2021
  • Revise Date: 10 March 2022
  • Accept Date: 26 September 2022