Document Type : Research Paper

Authors

• shabnam lotfi ¹
• mahdi behnamian ²
• sara Dezhsetan ³
• asghar estaji ⁴
• Younes Pour Beirami ⁵

¹ Department of Horticultural Sciences, University of Mohaghegh Ardabil

² Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

³ Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili

⁴ Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili

⁵ Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili,

Abstract

Iran is a country with a very diverse climate and rich in wild medicinal plants and mushrooms. Therefore, in order to use this diversity in breeding programs and also to preserve these natural resources, the study of genetic diversity is very important. In this study, the genetic diversity of 32 strains of Ganoderma lucidum collected from the northern strip of the country was investigated using 21 ISSR markers. According to the results, the polymorphic information content of markers ranged from 0.294 to 0.469 with a mean of 0.407. Also, Nei's genetic diversity, Shannon index and mean genetic distance were 0.214, 0.317 and 0.278, respectively. Based on principal components analysis, the first three components determined 63% of total variation. Based on cluster analysis, the investigated strains were divided into eight main groups. Sturcture analysis also revealed the presence of nine distinct genetic groups. In general, the results indicated that there was a favorable genetic diversity between the investigated strains of G. lucidum that can be considered in breeding programs.

Keywords

• Ganoderma
• Cluster analysis
• Polymorphism
• Sturcture analysis

Main Subjects

• Genetics