Evaluation of the effect of medium-term exercise on the response induced by thymrozal in the autophagy of the prefrontal part of male wistar rats

Document Type : Research Paper


1 1- Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, I. R. Iran.

2 Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences Branch, Islamic Azad University, Tehran, I. R. Iran.


Background and objective: In people with autism, communication, social interaction, and imaginative games have been particularly damaged. Thiomersal, commonly called mertiolate, is an organo-mercury compound that has antifungal and antiseptic properties. In recent years, the relationship between the likelihood of autism caused by thiomersal vaccines has been significantly raised by the media.

Methods: The present study examined dose-dependent behavioral changes after early exposure to thiomersal in a timed pattern of vaccination of children in male rats. Newborn mice were injected with 300 micrograms of thiomersal on days 7, 9, 11, and 15. From day 31 to day 80 After birth different exercise protocols 1 and 2 were applied. Immediately, after the respective protocols, in less than 30 seconds, the brain was removed from the skull and the brain prefrontal was rapidly exited. Finally, genes expression of ATG8 and ATG10 in the prefrontal l region were evaluated by Western blotting technique.

Conclusion: The results showed that the mice of receiving the thiomersal and did exercise protocols, especially the mice that did the 1 and 2 exercise protocol, had significantly lower ATG8 and ATG10 gene expression than the control group. Therefore, it can be concluded that mid-term exercise is effective in removing the adverse effects of thiomersal on the expression of autophagy genes.


Main Subjects

Volume 36, Issue 1
March 2023
Pages 28-45
  • Receive Date: 12 January 2021
  • Revise Date: 08 November 2021
  • Accept Date: 21 November 2021