Evaluation of silver nanoparticles effects on bla-per1 gene expression for biofilm formation in isolates of antibiotic-resistant Acientobacter Bumanni by real time PCR method

Document Type : Research Paper

Authors

1 azad shahrekord university

2 Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

3 Department of Biology,Faculty Of Science, Shahrekord Branch,Islamic Azad University,shahrekord,Iran

Abstract

Acinetobacter bomanni is one of the most common opportunistic pathogen in hospital that is resistant to many antibiotics due to the production of biofilm.
: In this experimental study, Acientobacter bumanni were isolates from 100 clinical samples. After identification of Acientobacter bummani strains and determination of antibiotic resistant profiles, biofilm producer isolates were determined using PCR method. The Minimum inhibitory concentration (MIC) of strains against AgNPs was determined. After 24 hours exposure of strains with subMIC concentration of AgNPs, RNA extraction and cDNA synthesis was performed. Finally, evaluation of bla-per1 gene expression was measured using real time PCR method.
Results: out of 100 clinical isolates, 12 isolates were belonged to Acientobacter bummani and all of strains were resistant to antibiotics except colistin. PCR results show that 12 isolates have bla-per1 gene and they were biofilm positive. Real Time PCR results show that after treatment of isolates with subMIC concentration of AgNPs, all of strains had a significant reduction in bla-per1 gene expression (P

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Volume 35, Issue 2
June 2022
Pages 349-366
  • Receive Date: 23 December 2019
  • Revise Date: 27 February 2020
  • Accept Date: 28 September 2020
  • First Publish Date: 16 December 2020