Document Type : Research Paper
Molecular markers have many applications in estimation of genetic diversity in crops and intron-exon splicing junction is a developed marker from RAPD that use as semi-random primers. In this research, 25 wheat genotypes including 23 rain-fed bread wheat cultivars/lines and 2 rain-fed durum genotypes were studied. Results showed that 17 primers from 32 semi-random primers had polymorphism among wheat cultivars/lines and percent of polymorphism varied from 38 to 88. Similarity matrices identified the highest similarity had between Maroon and Gahar cultivars. Cluster analysis on the basis of complete linkage method and Jacard similarity coefficient at 0.65 discriminated genotypes to 7 clusters and 2 genotypes were separated in 2 different clusters. Co-ordinate component analysis also could group the studied wheat genotypes based on their spatial distances. Although wheat cultivars/lines were well classified via 17 polymorphism primers, a low to medium genetic distance was estimated. This clustering could distinguish and isolate spring wheat cultivars/lines from winter ones and also hexaploid from tetraploid. Results showed that clustering via exon-targeting (ET) primers could distinguish and isolate winter wheat cultivars/lines from spring ones and durum wheat genotypes from hexaploid ones with a significant distance compared to intron-targeting (IT) primers.