نوع مقاله : مقاله پژوهشی
کلیدواژهها
موضوعات
عنوان مقاله English
نویسندگان English
Drought is a significant abiotic stress factor that impacts the productivity of crops, including wheat. However, drought tolerance is a complex trait involving numerous genes and cellular pathways. Understanding the molecular regulatory network of wheat in response to drought stress is crucial for molecular breeding. Non-coding RNAs affect plant growth and resistance to abiotic stresses by regulating gene expression. To help understand the complex regulatory mechanisms governing drought tolerance in wheat, we performed a total RNA transcriptome sequence analysis in a drought-tolerant wheat cultivar. A total of 20462 mRNAs and 209 miRNAs with different expressions were identified. Among 20462 genes with different expressions, 1025 were identified as genes that withstand drought stress. The target genes identified by psRNATarget were all enriched in gene ontology categories. Significant differences exist in the number of target genes regulated by differently expressed miRNAs, especially in signal transduction, stress response, and antioxidant pathways. Our results elucidate wheat's molecular mechanisms of drought tolerance and identify drought-responsive miRNAs and their target genes. These insights can be utilized to enhance wheat and related crops, providing valuable genomic resources for molecular breeding.
کلیدواژهها English