Document Type : Research Paper

Authors

• Ghasem Majdi Yazdi ¹
• Gholamhasan Vaezi ²
• Vida Hojati ³
• Mohammad Mohammad-Zadeh ⁴

¹ Teacher/The Ministry of Education

² Full Professor/Dept.of Biology,Damghan Branch,Islamic Azad University Damghan,IRAN.

³ Associate Professor/Dept.of Biology,Damghan Branch,Islamic Azad University Damghan,IRAN.

⁴ Psychiatry Research Center, ebne Sina Hospital, Mashhad University of Medical Sciences

Abstract

Background and Aim: The aim was to determine the effects of 6-ginger coated gold nanoparticles on the hippocampal level of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), oxidative DNA damage, and apoptotic indices in gold nanoparticles (AuNPs) affected rats.

Materials and Methods: 24 adult male Wistar rats were divided into 4 groups of 6. The animals in the control group received 0.5 ml of saline for 30 days (every other day). In the group of AuNPs, rats received 0.5 ml of gold nanoparticles (200 ppm and 60 nm) dissolved in saline, and after one month 0.5 ml saline for 30 days. In the Experimental Group 1, the same steps were performed in the AuNPs group, but instead of saline, the rats received 0.5 ml of gold nanoparticles coated with 6-gingerol (50 mg / kg). In the Experimental Group 2, similar steps to the AuNPs group were taken, but instead of saline, the animals received 0.5 ml of gold nanoparticles coated with 6-gingerol (100 mg /kg). At the end of the experiments, the level of hippocampal NGF, BDNF, 8-OHdG, and Bax and Bcl2 apoptotic indices were measured by ELISA.

Results: In the experimental groups, compared to AuNPs group, NGF, BDNF, and Bcl2 levels significantly increased, and Bax and 8-OHdG levels decreased significantly (P <0.001). However, all parameters did not reach the control group values.

Conclusion: 6-gingerol coated gold nanoparticles improve the hippocampal level of NGF and BDNF in rat exposed to gold nanoparticles by reducing apoptosis and oxidative DNA damage.

Keywords

• gingerol
• Nanoparticles
• Brain-Derived Neurotrophic
• Factor
• Nerve Growth Factor

Main Subjects

• Cellular biology