Document Type : Research Paper

Authors

• Gita vakili ¹
• Younes Pourbeyrami hir ²
• ‪Esmaeil Chamani ³
• asghar estaji ⁴

¹ Department of Horticultural Sciences, University of Mohaghegh Ardabil

² Ardabili Mohaghegh University, Dep. Of Hoyrticulture

³ Department of Horticultural Sciences, University of Mohaghegh Ardabili

⁴ Associate Professor, Department of Horticultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Tamarix is belonging to the Tamaricaceae family with 13 indigenous species of Iran and is one of the important ornamental plants in the landscape. This plant has been considered due to its resistance to the different weather and compatibility with salinity condition. In this study was conducted to investigate genetic diversity of different tamarix genotype endemic in the Ardebil province. The young leaves of 9 tamarix population were collected from natural habitats of this plant in Ardebil province to evaluate the genetic diversity. The leaves DNA were extracted using CTAB method. In this investigation 13 ISSR and 5 SCOT primers were used. A total number of 96 bands were produce by ISSR primers and the highest numbers of bands were amplified by UBC810- UBC826- UBC855. The average percentage polymorphism loci (PPL) was 6.75%, the highest polymorphism with the amount of 100% was in UBC814- UBC826 and UBC855 primers. The dendrogram was drowned based on WARD technique and genotypes classified into four main clusters. Also, a total of 32 band amplified by SCoT primer. The highest and lowest number of bands was obtained from SCoT4 and SCoT1, respectively. The average polymorphism percentage was 75.8%. In general, the results obtained from this study indicate that ISSR and SCoT primers were useful to separate the geographical region of genotypes. These primers also were highly efficient to determine the genetic diversity and relationship of studied tamarix populations. Hence, the use of these primers in other genetic studies like QTL and association mapping are recommended.

Keywords

• Cluster Analysis
• SCoT
• ISSR
• Tamarix
• Polymorphism

Main Subjects

• Genetics