Document Type : Research Paper

Authors

• maryam salavatifar ¹
• sheida Kashanpoor ²
• Ali Hatef Salmanian ³

¹ Department of Space Biology, Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran

² student

³ Faculty member/ NIGEB

Abstract

The pathogenic E. coli O157: H7 is capable of causing a wide range of complications, such as dysentery and hemolytic uremic syndrome. Due to the difficulties in antibiotic treatment, immunization seems to be the most appropriate way to fight this bacterium. Intimin, Tir, and EspA proteins are the three major proteins involved in the binding of the bacterium to the gastrointestinal tract. By inhibiting the binding of these proteins, colonization is limited and disease is inhibited. Selecting the effective portions of these proteins and producing a recombinant chimeric protein by binding them together, researchers have developed a suitable and effective immunogen to prevent the disease. Therefore, achieving high amounts of recombinant protein, along with maintaining its quality and efficiency, is very important. In the present study, due to the increase in the quantitative and qualitative expression of some recombinant proteins under gravity removal conditions, the expression of EIT recombinant proteins under simulated microgravity conditions on a clinostat device was investigated. After optimizing the expression conditions and confirming the recombinant protein using immunological methods, its expression was induced in weightlessness simulator conditions. After purification of the protein by chromatographic column, its amount was measured by standard methods. The results showed that despite the decrease in expression under weightlessness, probably due to lack of proper aeration of the culture medium, recombinant protein was produced with appropriate quality under weightlessness. It is expected that with optimizing the conditions and using aeration methods in weightlessness, the production of recombinant proteins to significantly increases.

Keywords

• E. coli O157: H7 bacteria
• Hemolytic uremic syndrome
• EIT recombinant protein Simulated microgravity
• Clinostat

Main Subjects

• Biotechnology