Iraninan Biology Society Journal of Cellular and Molecular Research (Iranian Journal of Biology) 2383-2738 38 2 2025 06 22 Optimization of Bacillus sp. AGh1‎‏ ‏culture medium for‏ ‏production of Endo-1,4-β-D-glucanase and the enzyme characterization Optimization of Bacillus sp. AGh1‎‏ ‏culture medium for‏ ‏production of Endo-1,4-β-D-glucanase and the enzyme characterization 127 142 2295 10.22034/cmr.2025.2295 FA Hasan Diba Biotechnology Department, Iranian Research Organization for Science and Technology ‎‎(IROST), Tehran, Iran, ‎ Jafar Hemmat Biotechnology Department, Iranian Research Organization for Science and Technology ‎‎(IROST), Tehran, Iran, ‎ Mohsen Vaez Biotechnology Department, Iranian Research Organization for Science and Technology ‎‎(IROST), Tehran, Iran, ‎ Arun Goyal Department of Biotechnology, Indian Institute of Technology Guwahati (IITG), Assam, India. 0000-0002-2741-9122 Journal Article 2023 07 26 Thermostable Endo-1,4-β-D-glucanase, known as a density reducer in cellulose ‎degradation, is one of the most widely used enzymes in industries. In this study, the ‎thermostability of Endo-1, 4-β-D-glucanase produced by Bacillus sp. AGh1 which was isolated from ‎a hot-spring in Ardabil province, Iran was evaluated. The enzyme production was optimized through two steps. Rice bran and yeast extract ‎were determination as the effective factors for enzyme production by Plackett-‎Burman method, and optimization of culture medium was performed by Response Surface ‎Methodology method. The amount of mentioned effective factors for producing the enzyme ‎were 58.8 and 14.87 g L-1, respectively. The optimum temperature and pH ‎for the enzyme activity were 70 ̊C and pH 5.6, respectively. Accordingly, Bacillus sp. ‎AGh1 can produce endo-1,4-β-D-glucanase enzyme from rice bran as cheap raw ‎material with suitable activity. Consequently, the enzyme has functional at the wide ‎range of pH and may be applied which needs more studies.‎ Thermostable Endo-1,4-β-D-glucanase, known as a density reducer in cellulose ‎degradation, is one of the most widely used enzymes in industries. In this study, the ‎thermostability of Endo-1, 4-β-D-glucanase produced by Bacillus sp. AGh1 which was isolated from ‎a hot-spring in Ardabil province, Iran was evaluated. The enzyme production was optimized through two steps. Rice bran and yeast extract ‎were determination as the effective factors for enzyme production by Plackett-‎Burman method, and optimization of culture medium was performed by Response Surface ‎Methodology method. The amount of mentioned effective factors for producing the enzyme ‎were 58.8 and 14.87 g L-1, respectively. The optimum temperature and pH ‎for the enzyme activity were 70 ̊C and pH 5.6, respectively. Accordingly, Bacillus sp. ‎AGh1 can produce endo-1,4-β-D-glucanase enzyme from rice bran as cheap raw ‎material with suitable activity. Consequently, the enzyme has functional at the wide ‎range of pH and may be applied which needs more studies.‎ https://cell.ijbio.ir/article_2295_16026d60ff9b54410b3435b403afd226.pdf