Iraninan Biology Society Journal of Cellular and Molecular Research (Iranian Journal of Biology) 2383-2738 37 3 2024 11 21 Choosing the optimal culture medium for seed germination and callus formation and determining the saponin content of the cell suspension of Acanthophyllum sordidum under salt stress Choosing the optimal culture medium for seed germination and callus formation and determining the saponin content of the cell suspension of Acanthophyllum sordidum under salt stress 272 288 2216 10.22034/cmr.2024.2216 FA Zahra Almasi Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Jalil Khara Associate Prof.- Department of Biology, Faculty of Science, Urmia University, Urmia, Iran 0000-0002-6773-1516 Mansour Afshar Mohammadian Department of Biology, Faculty of Sciences, Guilan University, Rasht, Iran Journal Article 2022 01 03 Abstract<br /><br />Acanthophyllum sordidum is a medicinal plant from Caryophllaceae family. This medicinal plant is one of the valuable sources of saponin. There are several methods to increase the production of plant secondary metabolites, among which tissue culture is used as an efficient technique. For this purpose, in the present research, to select the suitable culture medium for germination and calligenesis, to determine A. sordidum (Chubek) cell suspension growth curve, and also to determine the amount of saponin under salt stress in vitro, a factorial experiment was conducted in the form of a completely randomized design. The results showed that root length and root fresh weight were significantly increased in MS and WPM cultures, respectively. Other parameters including shoot length, total fresh weight and shoot fresh weight in the three culture medium used were not significantly different. Also, the results of analysis of variance of callus percentage from two leaf and stem organs did not show a significant difference among different culture media which have been used in this study (Ma, WPM and B5). Based on the current results obtained from the growth curve, the maximum growth rate was obtained 8 days after the start of suspension culture. Also, the results showed that salt stress caused a significant increase in saponin content of Chubek cell suspension. Abstract<br /><br />Acanthophyllum sordidum is a medicinal plant from Caryophllaceae family. This medicinal plant is one of the valuable sources of saponin. There are several methods to increase the production of plant secondary metabolites, among which tissue culture is used as an efficient technique. For this purpose, in the present research, to select the suitable culture medium for germination and calligenesis, to determine A. sordidum (Chubek) cell suspension growth curve, and also to determine the amount of saponin under salt stress in vitro, a factorial experiment was conducted in the form of a completely randomized design. The results showed that root length and root fresh weight were significantly increased in MS and WPM cultures, respectively. Other parameters including shoot length, total fresh weight and shoot fresh weight in the three culture medium used were not significantly different. Also, the results of analysis of variance of callus percentage from two leaf and stem organs did not show a significant difference among different culture media which have been used in this study (Ma, WPM and B5). Based on the current results obtained from the growth curve, the maximum growth rate was obtained 8 days after the start of suspension culture. Also, the results showed that salt stress caused a significant increase in saponin content of Chubek cell suspension. https://cell.ijbio.ir/article_2216_5938b4d054136e5d59ada6ec9c295d7a.pdf