Iraninan Biology Society Journal of Cellular and Molecular Research (Iranian Journal of Biology) 2383-2738 36 3 2023 09 23 Characterization and applications of plant peroxidase and nanoenzyme like-peroxidase Characterization and applications of plant peroxidase and nanoenzyme like-peroxidase 155 171 2165 FA Fahimeh Bahramnejad Department of Biotechnology, Institute of Science and High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran Ali Riahi-Madvar Department of Molecular and Cell Biology, Faculty of Basic Sciences, Kosar University of Bojnord, Bojnord, Iran Journal Article 2021 07 11 Peroxidases are one of the most applicable enzyem in the fields of industry, medicine and biotechnology. In this study, comprehensive information on the advantages and disadvantages and also applications of plant peroxidases and nanozymes (with peroxidase-like properties), are provided in a categorized manner using of numerous articles. Peroxidase enzymes are belong to the oxidoreductases group which are found in all living organisms and are classified into different classes, one of the most applicable of which is plant peroxidases. However, applications of peroxidases limit by the some factors such as high cost of purification, low stability and degradation by proteases. Todays, in order to overcome these problems, non protein substances or compounds with peroxidase-like properties, such as nanozymes, have been considered. For industrial applications, peroxidases can be provided by purification from various sources, and also their catalytic activities and stabilities can be optimized using various methods such as environmental engineering and protein engineering, whilest these methods are costly and time consuming. Peroxidase-like nanozymes can be prepared from various materials which their catalytic activities and stabilities can be optimized throuth changing of their size, shape and combination with other materials that are usually easy, fast and cheape. At the same time, problems such as storage and degradation by proteases, which are a challenge for enzymes, are not associated with the nanozymes. Overall, it seems in industrial and medical applications, plant peroxidases can replace by nanozymes. Peroxidases are one of the most applicable enzyem in the fields of industry, medicine and biotechnology. In this study, comprehensive information on the advantages and disadvantages and also applications of plant peroxidases and nanozymes (with peroxidase-like properties), are provided in a categorized manner using of numerous articles. Peroxidase enzymes are belong to the oxidoreductases group which are found in all living organisms and are classified into different classes, one of the most applicable of which is plant peroxidases. However, applications of peroxidases limit by the some factors such as high cost of purification, low stability and degradation by proteases. Todays, in order to overcome these problems, non protein substances or compounds with peroxidase-like properties, such as nanozymes, have been considered. For industrial applications, peroxidases can be provided by purification from various sources, and also their catalytic activities and stabilities can be optimized using various methods such as environmental engineering and protein engineering, whilest these methods are costly and time consuming. Peroxidase-like nanozymes can be prepared from various materials which their catalytic activities and stabilities can be optimized throuth changing of their size, shape and combination with other materials that are usually easy, fast and cheape. At the same time, problems such as storage and degradation by proteases, which are a challenge for enzymes, are not associated with the nanozymes. Overall, it seems in industrial and medical applications, plant peroxidases can replace by nanozymes. https://cell.ijbio.ir/article_2165_8929c70f8d710e412d38da624b21c3c8.pdf