Iraninan Biology Society Journal of Cellular and Molecular Research (Iranian Journal of Biology) 2383-2738 34 1 2021 04 21 Genome wide association study to identify genome region associated with methane emission in cattle using 30K panel Genome wide association study to identify genome region associated with methane emission in cattle using 30K panel 65 76 1613 FA Ali Jalil Sarghale Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R. of Iran Hossein Moradi Shahrbabak Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R. of Iran Mohammad Moradi Shahrebabak Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R. of Iran Ardeshir Nejati Javaremi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R. of Iran Mahdi Saatchi Department of Animal Science, College of Agriculture, Iowa State University, 806 Stange Road, Ames, IA 50011, USA Younes Miar Department of Animal Science and Aquaculture, Dalhousie University, Truro, B2N 5E3, Canada Journal Article 2018 12 27 The agriculture contribute to 50-60 % of global methane emission and ruminants have a key role in this contribution. Methane has an important role in global warming and it's global warming potential is 25 times greater than CO2. Furthermore, methane is the second most abundant global anthropogenic greenhouse gas after carbon dioxide. The present study aimed to perform genome wide association study to identify genome regions affecting the predicated methane production based on the concentrations of volatile fatty acids of rumen in Iranian Holstein cattle. One-hundred and fifty hair and rumen digesta samples were sampled using the two-tailed strategy based on breeding values of milk. After the measurement of the concentration of volatile fatty acids of rumen, methane emission of each animal was predicated according to these acids. DNA of animals was genotyped using GGP-LD v4 SNP panel (contains 30108 SNPs). Fourteen significant SNPs associated with methane emission trait located on 1, 2, 6, 10, 15, 17 and 18 chromosome were identified. Using annotating some quantitative trait locus (QTLs) were discovered around some of these SNPs. The results showed a genetic selection potential to mitigate methane emission per animal. Since genetic improvements are heritable, cumulative, and permanent, this improvement would be permanent and beneficial. The agriculture contribute to 50-60 % of global methane emission and ruminants have a key role in this contribution. Methane has an important role in global warming and it's global warming potential is 25 times greater than CO2. Furthermore, methane is the second most abundant global anthropogenic greenhouse gas after carbon dioxide. The present study aimed to perform genome wide association study to identify genome regions affecting the predicated methane production based on the concentrations of volatile fatty acids of rumen in Iranian Holstein cattle. One-hundred and fifty hair and rumen digesta samples were sampled using the two-tailed strategy based on breeding values of milk. After the measurement of the concentration of volatile fatty acids of rumen, methane emission of each animal was predicated according to these acids. DNA of animals was genotyped using GGP-LD v4 SNP panel (contains 30108 SNPs). Fourteen significant SNPs associated with methane emission trait located on 1, 2, 6, 10, 15, 17 and 18 chromosome were identified. Using annotating some quantitative trait locus (QTLs) were discovered around some of these SNPs. The results showed a genetic selection potential to mitigate methane emission per animal. Since genetic improvements are heritable, cumulative, and permanent, this improvement would be permanent and beneficial. https://cell.ijbio.ir/article_1613_69d658d0b2859e32cd4dc3b970c8496c.pdf