TY - JOUR ID - 649 TI - Identification of genetic loci controlling flowering time in oriental-type tobacco JO - Cellular and Molecular Research (Iranian Journal of Biology) JA - CMR LA - en SN - 2383-2738 AU - Darvishzadeh, Reza AU - hatami, hamid AD - Urmia university AD - Y1 - 2015 PY - 2015 VL - 28 IS - 3 SP - 438 EP - 447 KW - Composite interval mapping KW - genetic population KW - interval mapping KW - microsatellite DO - N2 - In order to identify genetic loci associated with flowering time in oriental-type tobacco, the genetic population comprising 100 F2 individuals from the cross between two oriental-type genotypes Basma Seres 31 (maternal) × SPT 406 (paternal) were evaluated for days to flowering character. In molecular experiment, linkage map with 23 SSR and 29 ISSR markers were prepared which covered 570/8 cM of tobacco genome. Using interval and composite interval mapping procedures, 9 and 2 QTLs were identified for studied character, respectively. In this study, the most identified QTLs were located on linkage group 5. Genetic loci qDF5-3 and qDF2-27, identified via interval mapping, with 0.8 and 36 percent of R2 were the minor and major QTLs, respectively. According to results, the percentage of phenotypic variance (R2) explained by identified QTLs through composite interval mapping (qDF5-1 and qDF5-2), ranged from 1.95 to 15.34. Results revealed the role of both additive and dominance effects in genetic control of days to flowering in oriental-type tobacco. UR - https://cell.ijbio.ir/article_649.html L1 - https://cell.ijbio.ir/article_649_1819d89b56d723abe01ce1d509d51dd0.pdf ER -