TY - JOUR ID - 1624 TI - Using olive methanolic extracts to inhibit rotenone toxicity in Parkinson’s cell model JO - Cellular and Molecular Research (Iranian Journal of Biology) JA - CMR LA - en SN - 2383-2738 AU - Morshedi, Dina AU - Aliakbari, Farhang AU - Parsafar, Soha AU - Mohammad-Beigi, Hossein AU - Dehghani Esmatabad, Faezeh AU - Amiri-Nowdijeh, Alireza AD - Faculty member, department of industrial biotechnology, National Institute of genetic engineering AD - National Institute of Genetic Engineering and Biotechnology, Tehran, I.R. of Iran AD - National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AD - Interdisciplinary Nanoscience Centre (iNANO) and Department of Molecular Biology and Genetics, Aarhus University AD - , National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AD - National institute of genetic engineering and biotechnology, Tehran, Iran Y1 - 2021 PY - 2021 VL - 34 IS - 2 SP - 246 EP - 256 KW - Parkinson KW - rotenone KW - cytotoxicity KW - olive extract DO - N2 - Abstract: Central nervous system disorders, including Parkinson's Disease (PD), are growing pathogenic incurable diseases. In PD, with the loss of neurons especially in the areas which produce and secrete dopamine, the patient is facing with irreversible motorized and perceived complications. Neuronal damage and death are usually accompanied by increasing the expression of alpha-synuclein (αSN) protein. Rotenone, a known neurotoxin, is also contributed to PD by inducing oxidative stress. In this study, we investigated the effect of the extracts of three Iranian olive cultivars on the PD cell model which overexpresses αSN. These cells react strongly to rotenone through which, above and beyond neurotoxicity, the length of the neurites is also reduced in the treatment with rotenone. Some extracts played a protective role in rotenone-treated cells and decreased intracellular reactive oxygen species (ROS) significantly. The radical scavenging activity of the extracts suggested that these reagents can play a protective role on neuronal cells through this mechanism; however, due to the different behavior of the extracts in the extracellular environment, it seems that more complex mechanisms are involved in their neuronal protective effect. This study showed that original Iranian olives belong to Roghani and Zard cultivars could protect the neurons against damage adopted from toxins and αSN and finally could be used in the diet to prevent PD. UR - https://cell.ijbio.ir/article_1624.html L1 - https://cell.ijbio.ir/article_1624_278962e221ceb516d91628c6c2d728b2.pdf ER -