Biological Production of Natural Vanillin Based on the Microbial Conversion of Phenylpropanoids
Morahem
Ashengroph
Asistant Professor
author
Iraj
Nahvi
عضو هیات علمی دانشگاه اصفهان
author
text
article
2014
per
The rising popularity of natural aroma products has triggered off significant research activities to use biocatalysts for the production of natural flavor compounds. Vanillin is of major interest for the flavor and fragrance industry. It has been widely used as a flavoring agent in foods, perfumes, beverages, pharmaceuticals and medical industries. Two main approaches for the production of natural vanillin are direct extraction from botanical sources and microbial bioconversion. Considering the various applications of vanillin and increasing consumer-led demand for natural vanillin and also due to the fact that the extraction from botanic sources is very time consuming, expensive and does not satisfy the worldwide demand, alternative biotechnological methods for its production are being constantly explored.Microbial bioconversion has been extensively exploited to make modifications in the structure of the organic compounds. Microbial cells can be efficiently used as a eco-friendly biocatalyst in the conversion of substrates as precursors into value-added products. The current review emphasizes the microbial bioconversion routes involved biological production of natural vanillin based on the microbial conversion of henylpropanoids.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
316
334
https://cell.ijbio.ir/article_507_288c28ac8c5e74d6dfc84f6d525d1edd.pdf
dx.doi.org/2731
Presentation of Novel Strains of Halophile Bacteria Salinivibrio sp. from Urmia Lake
عباس
اخوان سپهی
دانشگاه آزاد اسلامی
author
شبنم
ایران نژاد
دانشگاه آزاد واحد تهران شمال
author
محمد علی
آموزگار
آزمایشگاه اکستریموفیل ها، گروه میکروبیولوژی، دانشکده زیست شناسی، پردیس علوم، دانشگاه تهران
author
امیر
توکمه چی
گروه پاتوبیولوژی و کنترل کیفی، پژوهشکده آرتمیا و جانوران آبزی، دانشگاه ارومیه
author
text
article
2014
per
In this research, for the first time identification of moderate halophile microorganism of the genus Salinivibrio was studied, which leaded to identification of novel strains of this genus. Urmia Lake is a hypersaline lake located at the north-west region of Iran and it is the saltiest perennial lake of Iran and one of the rare perennial hyper saturation lakes in the world.At first, samples were collected from different sites of Urmia Lake and transferred to the laboratory under sterile conditions. After enrichment and culture of samples under defined conditions, repeated cultures were carried out to achieve pure cultures. Then selected strains were studied regarding phenotypic and phylogenetic (by sequencing 16S rRNA technique) characteristics. From total strains which were belonged to Salinivibrio sp. , 57% and 43% of the strains showed closest similarity with S. costicola subsp. alcaliphilus and S. sharmensis , respectively.This study is attempted to present of novel genetic pools from great microbial sources of Urmia Lake and especially Salinivibrio sp. that they are the base of wide applications in biotechnological science.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
335
345
https://cell.ijbio.ir/article_443_2476f9a07ec6cc58a5a4e4268c2bf637.pdf
dx.doi.org/2732
Treatment of oily wastewaters by Lipase enzyme producing bacteria
ابوالفضل
اژدرپور
دانشگاه تربیت مدرس
author
bagher
Mortazavi
استاد دانشگاه تربیت مدرس
author
غلامرضا
موسوی
استاد دانشگاه تربیت مدرس
author
text
article
2014
per
AbstractOily Wastewater causes severe environmental pollution and damage to aquatic life. Different methods for treating the wastewater are applied to one of the most important biological methods. Enzymatic treatment of wastewater is also an example of the ways in which biological research has attracted much attention. The purpose of this study, isolation of bacteria producing lipase enzyme and then use them in the oily wastewater treatment. Two separate locations, including Abadan refinery soil and the Behshar Industrial town sewage treatment plant. Among the isolated bacteria, two strains of bacteria belonging to Staphylococcus and Flavobacter was used for subsequent studies. They ranged between 0.6 to 3.25 u/ml of lipase activity. The removal of the oil was measured using gravimetric, for concentrations less than 4 grams per liter to about 95% and for concentrations greater than 4 gr/l ranged from 20 to 50 percent.Keyword: wastewater treatment, oil, lipase, bacteria .
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
346
353
https://cell.ijbio.ir/article_435_47e428ccc4a63ab690a142c8f78f2054.pdf
dx.doi.org/2733
Design a biosensor to measure glucose by using modified electrode with cadmium oxide nanoparticles and glucose oxidase enzyme
سعید
رضایی زارچی
دانشگاه پیام نور یزد
author
masoud
negahdary
دانشگاه آزاد اسلامی واحد مرودشت، باشگاه پژوهشگران جوان، مرودشت، ایران
author
text
article
2014
per
In this study, we introduced a new biosensor for measurement of glucose by using of glucose oxidase enzyme, cadmium oxide nanoparticles and carbon paste electrode. Size of cadmium oxide nanoparticles were about 43 nm. The results indicated that produced carbon paste electrode modified with cadmium oxide nanoparticles, can facilitates electron transfer in stabilized glucose oxidase enzyme on the electrode surface. In addition, the reduced form of glucose oxidase enzyme could oxidize by solutions oxygen with an electro catalytic reaction, that this oxidation, due to reaction between the oxidized form of glucose and glucose oxidase and this reaction inhibited by glucose. Based on electro catalytic decrease of glucose oxidase enzyme in saturated oxygen solution in the presence of glucose, a new sensor was designed for glucose. Designed sensor has high sensitivity and in linear range of 20 µM to 360 µM and can be used to determine glucose. This biosensor also has very good stability.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
354
366
https://cell.ijbio.ir/article_433_50f1c79dc057ef2de3bebe5a5a4ead8e.pdf
dx.doi.org/2734
Teratogenic and cytotoxic effects of Salen, A current ligand in vanadium complexes
Saber
Zahri
University of MOhaghegh Ardabili
author
Abolfazl
Bezaatpour
University of Mohaghegh Ardabili
author
Arash
Abdolmaleki
University of Mohaghagh Ardabili
author
text
article
2014
per
Metal salen complexes have been widely studied as potentially pharmaceutical substances with antioxidant and anti cancer effects. In this investigation the salen ligand was synthesized and injected in the air sac of the eggs. The liver and fibroblast cell cultures were treated by the compound and the proportion of the live cells was recorded. The survival fraction of the embryos was 23.5% in the uppermost concentration of the compound and the LD50 was 91.8 μM/egg. The morphological studies showed clubfoot, the abnormality of beak and ectopic viscera, and the skeletal staining showed the deletion and unosification of the caudal vertebrates, the brevity and the bending of tibia. The compound inhibited the growth of liver and fibroblast cells with the IC50 of 1159.3 and 964.7 μM, respectively. The treated cells were round, the inter-cellular connection became loose, the proliferation was inhibited, and the granules in the cytoplasm were increased. The compound significantly affected the proliferative cells compared with the cells were arrested in growth.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
367
376
https://cell.ijbio.ir/article_439_a949fb4e2b6af3c38b515dccafc30d3f.pdf
dx.doi.org/2735
Molecular Cloning of Plasma Membrane H+-ATPase Coding Sequence from Aeluropus littoralis Halophyte Grass
احسان
شکری
پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان
author
Najmeh
Nasiri
دانشگاه علومکشاورزی و منابعطبیعی ساری
author
قربانعلی
نعمت زاده
پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
text
article
2014
per
H+-ATPase proton pump is one of the major proteins found in plasma membrane of plants which plays an important role in molecular physiology of stress response. Aeluropus littoralis grass due to resistance to some environmental stresses like salinity and heavy metals is a good model for studying the structure and function of the plasma H+ATPase pump. In this study as a starting point, the complete coding sequence of the gene were identified and cloned from Aeluropus littoralis halophyte. Preliminary data showed that nucleotide sequence of A. littoralis plasma membrane proton pump contains a 2856 bp full-length ORF region that shared a significant homology with Sorghum bicolor ، Oryza sativa and Zea mays. Also the predicted peptide of this gene consists of 165 amino acid residues with a molecular weight of 104567 Da which is in terms of domains type and structure, consistent with most plant and fungal plasma membrane H + ATPase protein type III (type III) from a large family pump ATPase p-type. Comparative alignment of amino acid sequence of A.littoralis plasma membrane H+ATPase with some species indicated that nucleotide substitutions did not targeted critical and functional positions of protein and these regions remained conserved. At last the sequence was submitted to GenBank with accession number of GI: 346230720.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
377
388
https://cell.ijbio.ir/article_437_d667499f25fc62ea1d5b76eda3dcde11.pdf
dx.doi.org/2736
Isolation and identification of two laccase producer fungi from bagass and sugarcane rhizosphere
Fatemeh
Sheikhi
موسسه تحقیقات و آموزش توسعه نیشکر
author
Mohammad
Roayaei Ardakani
استاد-دانشگاه شهید چمران اهواز
author
Naimeh
Enayatzamir
استاد- دانشگاه شهید چمران اهواز
author
Gholamreza
Ghezelbash
دانشگاه شهید چمران اهواز
author
text
article
2014
per
AbstractComplex of enzymes in the name of Ligninase are involved in lignin degradation. Special properties of Laccase among the lignin degradation enzymes make them more important. Oxidation of wide range of substrates, high heat tolerance and acidic isoelectric pH are main properties of laccase. Identification of new microorganism with higher production and secretion laccase is very valuable. In this research, laccase producing fungi was isolated, screened and identified from bagass and sugarcane rhizosphere. In order to screen laccase producing strains malt extract agar medium containing 0.1gl-1 ABTS or 0.5mMl-1 Guaiacol was used. Laccase activity was determined as described by Niku-Paavola et al. In this study, among 22 fungi screened, Alternaria alternata and Retroconis sp isolates were identified with the highest laccase production. Two species were identified using PCR amplification and sequencing of the internal transcribed spacer ‘ITS’regions of the ribosomal DNA and by basic morphology. In this research laccase activity has been determined in agitation and stationary state. These results showed that agitation and stationary conditions had different effects on laccase production rate of these isolates.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
389
398
https://cell.ijbio.ir/article_440_8f9b609b6fc564d236aefb5578b8589d.pdf
dx.doi.org/2737
Induced apoptosis of bladder carcinoma cell line 5637 by podophyllutoxin treatment
Eman
Sadeghi
دانشگاه تربیت مدرس
author
mehrdad
behmanesh
tarbiat modaresuniversity
author
Mozafar
Sharifi
دانشگاه تربیت مدرس
author
bahram
mohammad soltani
دانشگاه تربیت مدرس
author
najmeh
ahmadian
دانشگاه تربیت مدرس
author
text
article
2014
per
Chemotherapy has been used for centuries in cancer therapy. Different compounds and drugs are used in chemotherapy; each is applied for a specific type of cancer. Among them; plant compounds including lignans, have been used in medicine for a long time. Podophyllutoxin is a member of lignans family that is mostly extracted from Podophyllum species. It has multiple biological properties such as antiviral, antifungal and anticancer properties. Considering anticancer property of podophyllutoxin, it is used in Wilm`s tumor and genital tumors treatment. Also, it has been shown that podophyllutoxin causes apoptosis in ovary carcinoma cell line (Hela). There is not yet any report of the effect of podophyllutoxin on bladder carcinoma cell line such as 5637. In this study, we have evaluated the effect of podophyllutoxin on cell viability and apoptosis of 5637 cell line, and we have shown that podophyllutoxin causes apoptosis and death of these cells. It seems that podophyllutoxin can be an active agent to induce apoptosis of cancer cells.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
399
405
https://cell.ijbio.ir/article_438_ab5cf5a9c37963d53b79944146d39f0f.pdf
dx.doi.org/2738
The study of microbial removal efficiency of crude oil in contaminated soils of cold climates areas (case study: Tabriz refinery)
Fariba
Mohsenzadeh
عضو هیات علمی دانشگاه بوعلی سینا
author
text
article
2015
per
Petroleum pollution is the most important soil pollution. Bioremediation is an effective and usable technology for remediation of polluted soils. Some microorganisms have bioremediation potency for petroleum compounds. In this research Tabriz city was selected as a cold area of Iran for studying of native bacteria with biodegradation potency of crude oil. Soil samples were collected from polluted soils of Tabriz refinery and their bacteria were accommodated to crude oil. The bacterial strains exited in the samples were isolated, grouped and determined. The growth ability and petroleum removal efficiency were evaluated for the each bacterium at the different crude oil concentrations during a month. In this research, seven bacterial species were found that are well accommodated to crude oil. The highest petroleum removal efficiency (98%) was determined for Pseudomonas aerogenesis at concentration of 0.5%. Biological removing of crude oil with high efficiency is possible by soil bacterial flora, as an environmental friendly method, even for soil cleaning of the industrial areas in cold temperature regions.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2015
406
417
https://cell.ijbio.ir/article_510_9dd1e893610bb9d2d1e773a7fefef023.pdf
dx.doi.org/27313
Effects of feeding strategy on reproductive performance and ovarian morphology of broiler breeder hens
مازیار
محیطی اصلی
دانشگاه تهران
author
محمود
شیوازاد
عضو هیات علمی دانشگاه تهران
author
مجتبی
زاغری
عضو هیات علمی دانشگاه تهران
author
مریم
رضائیان
عضو هیات علمی دانشگاه تهران
author
Saeed
Aminzadeh
Academic Staff
author
text
article
2014
per
The effects of feeding strategy of broiler breeder hens on reproductive performance and ovarian morphology from 42 to 54 week of age were studied with using 50 Ross 308 broiler breeder hens. Hens were randomly divided to 2 groups of restricted (160 g/d) and ad libitum (208 g/d) feeding. Ad libitum fed hens had 630 g heavier body weight than restricted hens. Egg production rate decreased 23.3% during the first 2 weeks of experiment in ad libitum fed hens compared to restricted hens. Egg weight was increased quickly during first 5 week of experiment in ad libitum fed hens and then continued slowly. Percentage of fertility and hatchability significantly decreased in ad libitum fed hens compared to restricted hens (P≤0.05). Ad libitum fed hens had more atretic and third follicles (P≤0.05). However, there were no significant difference in ovary weight between the two feeding group, oviduct weight was lower in ad libitum fed hens (P≤0.05). Abdominal fat pad weight, liver weight, percentage of liver lipids and cholesterol was higher in ad libitum fed hens compared to restricted hens (P≤0.05). Microscopic examination of liver tissue revealed huge vacuoles of fat which was not detected in restricted hens. Results indicate that ad libitum feeding of broiler breeder hens by increasing of fat deposit in abdomen and liver, reduce reproductive performance.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
418
427
https://cell.ijbio.ir/article_434_106fb3b08f91393300200683115fc383.pdf
dx.doi.org/27310
A genetic approach to accurately predict RNA secondary structure
soheila
montaseri
Tarbiat Modares University
author
Nasrollah
Moghadam Charkari
Tarbiat Modares University
author
Fatemeh
Zare Mirakabad
Amirkabir University of Technology
author
text
article
2014
per
RNA molecule plays important and fundamental roles in many biological processes. In the most times, activities of RNAs are determined by their structures. In notice to complexity and costly of laboratory methods to predict RNAs structure, computational approaches are used. There are variety of algorithms to predict RNA secondary structure. In this paper, a genetic algorithm called RNAG is presented to predict the RNA secondary structure based on minimum free energy (MFE). In this algorithm, each individual of population includes some stems. The individuals are increasingly ranked based on fitness value of MFE from stems and loops, and in the follow, crossover and mutation operations are done on individuals to make a new population, respectively. Process of population generation continues until an individual with proper MFE is produced. Finally, this individual is selected as an optimal RNA secondary structure. The proposed algorithm is performed on some RNAs in the bacteria. Results of the paper show that RNAG algorithm has a high accuracy in comparison with the other related methods.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
428
437
https://cell.ijbio.ir/article_436_2a734b08b9ecab4bedfbee20083697f9.pdf
dx.doi.org/27311
Quantitative Study of the Genes Involved in Survival and Autophagy Pathways in T-47D Cell Line with Emphasis on Upgrading Cold Resistance of the Cells in the Presence of DMSO
Mahrou
Vahabi
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
author
Shahrokh
Safarian
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
author
Seyed Jalal
Zargar
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
author
Laya
Aliasghari
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
author
text
article
2014
per
Dimethylsulfoxide (DMSO) is one of the cryoprotectants utilizing in freezing and long term maintenance of the cells. In this study, the mechanism of protection of this mater in relation with cell death and autophagy is envisaged. Study of the expression level of autophagic genes (atg5، lc3، beclin1، dram و p53), using real time RT-PCR, indicated that at the lowest concentration of DMSO the genes involved in autophagy are over-expressed, thus regarding to death autophagy induction, the cells have lower viability relative to those cells which are freezed at the higher concentrations of cryoprotectant. Increasing cell viability, at the greater concentrations of DMSO (over than 10%), occurs because of the converting of death autophagy to cytoprotective one which is supported by means of over-expression of the genes involved in the survival pathway. Simultaneous study of autophagy and AKT/mTOR pathway can be utilized as a separating method for death and survival autophagy.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
438
452
https://cell.ijbio.ir/article_444_402b3ca75eb3b6de953f4eb6d92fcf88.pdf
dx.doi.org/27312
Lab and In silico study of expression and aggregation of recombinant core protein of Hepatitis C virus in Escherichia coli
Jafar
Hemmat
Faculty- Iranian Research Organization for Science and Technology
author
Bagher
Yakhchali
عضوهیئت علمی- پژوهشگاه ملی مهندسی ژنتیک
author
َAliashgar
karkhaneh
عضو هیئت علمی- پژوهشگاه ملی مهندسی ژنتیک
author
mahnaz
mazaheri asadi
عضو هیئت علمی- سازمان پژوهش های علمی و صنعتی ایران
author
soodabeh
karimi
سازمان پژوهشهای علمی و صنعتی ایران
author
text
article
2014
per
In this study, expression of mature Hepatitis C virus core protein (HCVC173), consistent with a viral isotype isolated in Iran, was studied in E. coli. The 20 kDa- HCVC173 protein was expressed while it contains 18% of total expressed proteins in which a part was partially soluble but the main part was expressed in insoluble inclusion body. However, the produced inclusion body has not shown the expected purity and HCVC173 has been aggregated with the host proteins. Moreover, during refolding of insoluble HCVC173, notable aggregation of the protein was undesirable phenomena. Intramolecular evaluation of target expressed protein predicted two irregularity area in the HCVC173 which may aggregate the expressed protein. Consequently, HCVC173 has the features of the intrinsically unstructured proteins. This character may justify its aggregation during refolding. Moreover, the approach presented in this paper is an alternative solution to overcome the expression limitation of mature form of the HCVC protein.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
453
461
https://cell.ijbio.ir/article_441_f05202c54810d17fd7fef8c68b31b7eb.pdf
dx.doi.org/27313
Comparison of intron and exon markers in assessment of genetic diversity between Thymus pubescens
فرزانه
مجیری
دانشگاه لرستان
author
احمد
اسماعیلی
عضو هیأت علمی دانشگاه لرستان
author
farhad
nazarian firoozabadi
دانشگاه لرستان
author
رضا
مؤمنی
دانشگاه زابل
author
text
article
2014
per
Thymus pubescens is one of the medicinal plants of Lamiaceae, growing endemically in most regions (especially in north and west) of Iran. Molecular markers were used in genetic diversity studies of medicinal plants for breeding applications. In this study, 30 semi-random primers, including IT (intron targeting) and ET (exon targeting), were used for assessment of genetic diversity among Thymus pubescens accessions. DNA extracted from fresh leaves of Thyme and polymerase chain reaction products were separated on 1.5% agarose gel by electrophoresis. All of primers produced 559 bands which 96% of them were polymorphic. 302 and 257 polymorphic bands belong to IT and ET primers, respectively. The highest polymorphic information content (PIC) and marker index (MI) among IT and ET primers belonged to IT18-1 and ET12-27, respectively. Cluster analysis 0f IT and ET primers, using UPGMA method, Dice's similarity coefficient and DARwin5 software, divided accessions into three and four clusters, respectively. In both dendrogram, the highest genetic similarity showed between two accessions with similar geographical origin. Cluster analysis results were aligned with grouping of principal component analysis. Results of this study revealed that IT primers could discriminate Thymus pubescens accessions in compared with ET primers.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
27
v.
3
no.
2014
462
472
https://cell.ijbio.ir/article_502_e51f0cc4ffe31144fcbbfa3d9fd98278.pdf
dx.doi.org/27314