Evaluation of three Satureja species essential oil (S. macrantha, S. rechingeri, S. spicigera) against bacteria that cause hospital infections and Candida albicans
elmira
ehsani
Islamic Azad University
author
Fatemeh
Sefidkon
Research Institute of Forest and Rangeland, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. of Iran
author
farzaneh
Hoseini
Islamic Azad University
author
text
article
2017
per
Abstract:In order to evaluation of three Satureja species essential oils (S. macrantha, S. rechingeri, S. spicigera) against the bacteria that cause hospital infections and Candida albicans, in the first two accessions of every three species collected in 1391 from The Botanical Garden of Research Institute of Forests and Rangelands, the essential oil was isolated by water distillation .analyzed constituents of essential oils by GC/MS and the results show the essence of Satureja macrantha and Satureja spicigera, 16 components and essence of the Satureja rechingeri, 15 components were identified. The major compounds in the essential oils were Thymol, Carvacrol, p-cymene and -terpinene.In this experiment , essence dilution was in three levels including one-fifth, one twenty-fifth and one fiftieth, and compare them with antibiotics (Ciprofloxacin and Ceftizoxime) and micro organisms at 5 levels , including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.The antibacterial activity was determined by disk diffusion method and estimate against five species. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oils were determined with microdilution. Pseudomonas aeruginosa was the most resistant bacteria and Candida albicans Was more sensitive than bacteria. Satureja macrantha Showed the highest antimicrobial activity and Satureja spicigera showed the lowest antimicrobial activity.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
107
119
https://cell.ijbio.ir/article_928_1ccecdc759e7dcc3c0ecd4d9ed23a2f7.pdf
Assessment of genetic diversity among rain-fed wheat genotypes, using intron-exon semi random primers
احمد
اسماعیلی
عضو هیأت علمی دانشگاه لرستان
author
فرهاد
نظریان فیروزآبادی
دانشگاه لرستان
author
کامران
سمیعی
دانشگاه لرستان
author
رضا
دریکوند
دانشگاه آزاد اسلامی واحد خرمآباد
author
text
article
2017
per
Molecular markers have many applications in estimation of genetic diversity in crops and intron-exon splicing junction is a developed marker from RAPD that use as semi-random primers. In this research, 25 wheat genotypes including 23 rain-fed bread wheat cultivars/lines and 2 rain-fed durum genotypes were studied. Results showed that 17 primers from 32 semi-random primers had polymorphism among wheat cultivars/lines and percent of polymorphism varied from 38 to 88. Similarity matrices identified the highest similarity had between Maroon and Gahar cultivars. Cluster analysis on the basis of complete linkage method and Jacard similarity coefficient at 0.65 discriminated genotypes to 7 clusters and 2 genotypes were separated in 2 different clusters. Co-ordinate component analysis also could group the studied wheat genotypes based on their spatial distances. Although wheat cultivars/lines were well classified via 17 polymorphism primers, a low to medium genetic distance was estimated. This clustering could distinguish and isolate spring wheat cultivars/lines from winter ones and also hexaploid from tetraploid. Results showed that clustering via exon-targeting (ET) primers could distinguish and isolate winter wheat cultivars/lines from spring ones and durum wheat genotypes from hexaploid ones with a significant distance compared to intron-targeting (IT) primers.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
120
129
https://cell.ijbio.ir/article_1086_706ccb61e1078347e7db58386458b2f6.pdf
Effect of low temperatures on the total protein, proline and the activity of some antioxidant enzymes of Stevia rebaudiana Bertoni
Mansour
Afshar Mohammadian
Academic Staff
author
zahra
ansari
دانشگاه گیلان
author
text
article
2017
per
Nowadays, natural sweeteners, especially for people with diabetes have attracted great attention. Stevia rebaudiana,Asteraceae, have Glycoside terpens including Stevioside, Dudoside and Rebaudioside which are 300 times sweeter than sucrose is very important, economically and scientifically. To investigate the impact of low temperatures on the total protein, proline and the activities of some enzymatic antioxidants of stevia, a completely random factorial design experiment with three levels of temperatures 25 (control), 15 and 5°C in three replications was conducted. According to the results, stevia leaves showed no significant differences in total protein, proline content and the activity of the examined antioxidant enzymes including SOD, APX, POD, PPO, under low temperatures of 15 and 5°C. Therefore, it can be concluded that the temperatures of 15 and 5°C cannot create stress for Stevia plants, and so probably this plant is physiologically tolerant at this cold levels. Also, apparently the treated plants were not significantly different compared to controls
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
130
140
https://cell.ijbio.ir/article_971_e7fdb3d955ab81fad6508e4da4c42bc7.pdf
Grouping and estimation of genetic diversityof different ecotypes of medicinal plant of Plantago psyllium using ISSR marker
مهدی
رمضانی
باشگاه پژوهشگران جوان و نخبگان، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران.
author
Mahdi
Rahimi
Biotechnology Dept., Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. of Iran
author
text
article
2017
per
The genetic variations of 17 ecotypes of sand plantain were evaluated by 12 ISSR markers and nine morphological traits. Analysis of variance showed high variability among studied cultivars. Cluster analysis could put 17 different ecotypes of sand plantain into the three groups using UPGMA method based on field data. The assessment of ecotypes based molecular markers showed that the 12 primers could be amplified 91 polymorphic bands, the maximum number (14) was produced by UBC814 and primers UBC811, UBC813 and UBC817 with 13 bands were in the next steps respectively. The minimum band number (7) was produced by UBC824 and UBC876 respectively. PIC value was varied from 0.27 to 0.44 and MI was 0.91 to 4.10. Cluster analysis using UPGMA based molecular markers, placed 39 ecotypes in the study in five groups, include 3, 9, 3, 1 and 1 ecotypes respectively. Grouping of ecotypes with molecular markers is moderate matched with classification of the ecotypes based morphological traits.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
141
151
https://cell.ijbio.ir/article_1080_876387ac2c9b1ab356981765a731e0ed.pdf
Effect of methyl jasmonate, mevalonic acid and potassium nitrate in artemisinin content by suspension cultures of Artemisia annua L.
mandana
samimizad
kharazmi
author
پریسا
جنوبی
دانشگاه خوارزمی
author
محمدرضا
نقوی
دانشگاه تهران
author
الیاس
آریا کیا
ibrc
author
بنیامین
یزدانی
دانشگاه محقق اردبیلی
author
ابوالحسن
شاهزاده فاضلی
ibrc
author
text
article
2017
per
Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L. ( Asteraceae), is the most effective antimalarial Drug, It is also effective against a wide variety of cancers. Due to the low content of artemisinin in A. annua leaves and uneconomical chemical synthesis, tissue culture is essential. The purpose of this research is to focus the application of tissue culture technology and the cell suspension culture to increase artemisinin by elicitors. Callus cultures were induced from root explants at different BA and NAA concentrations in MS medium. The best callogenesis was on BA (1.5 mg/l-¹) and NAA (0.5 mg/l-¹). Suspension culture were initiated by transferring friable fraction of root callus into liquid MS medium with BA (1.5 mg/l-¹) and NAA (0.5 mg/l-¹). In an effort to increase the artemisinin production, methyl jasmonate, mevalonic acid lactone and potassium nitrate with different concentrations was tested by treating Suspension culture. A large increase in artemisinin content was found in 5 mg/l-¹ methyl jasmonate, 75 mg/l-¹ mevalonic acid lactone and 750 mg/l-¹ potassium nitrate. It seems suspension cultures of Artemisia annua is Effective in artemisinin Production. Also the addition of elicitors increase artemisinin content.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
152
160
https://cell.ijbio.ir/article_902_b7e5de922382623e09db3fa0f622d2db.pdf
Synthesis of Silver Nanoparticles Using Green Method of Plant Extract European Marjoram (Origanum majorana) and Their Antibacterial Effects
Somayyeh
kavoosi
گروه زیست شناسی، واحد اردبیل، دانشگاه آزاد اسلامی
author
hashem
yaghoubi
گروه زیست شناسی، واحد اردبیل، دانشگاه آزاد اسلامی
author
text
article
2017
per
There are many different methods for the production of silver nanoparticles (Ag-NPs) but, using of plants in the synthesis of nanoparticles due to a cost effective and eco-friendly approach has been extensively noticed. In this study, the biosynthesis of silver nanoparticles by using extract of Origanum majorana and its antimicrobial properties has been reported. In this study, the extract of Origanum majorana as the reducing agent is used for the biosynthesis of silver nanoparticles. Reduction of Ag+ ion was obtained after 24 h by using extract of Origanum majorana in presence of 1 mM silver nitrate (AgNo3) solution. The size, structural, optical and morphological properties of nanoparticles have been analyzed by facility of: X-ray Diffraction, Field Emission Scanning (Transmission) Electron Microscope. The antimicrobial activities of the silver nanoparticles against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of biological Ag nanoparticles. Size and morphological properties of nanoparticles was performed by TEM which show that particles have spherical shape with diameter of about 30-70 nm. Silver nanoparticles presented the antimicrobial activity against Gram positive and Gram negative bacteria. It seems that green Synthesis of nanoparticles using plant extracts can help to increase their antibacterial. In the present study it was shown that the antibacterial properties of nanoparticles to increase considerably.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
161
173
https://cell.ijbio.ir/article_1122_841d28a060f7f41df4f9755a7589a71e.pdf
Electricity production in two-chamber microbial fuel cells using exoelectrogenic Shewanella sp. isolated from sediments of the Caspian Sea
Mojtaba
Mohseni
University of Mazandaran
author
سیده مریم
اکرامی
دانشجوی کارشناسی ارشد میکروبیولوژی
author
text
article
2017
per
A microbial fuel cell is a bioelectrochemical system that converts chemical energy in organic compounds to electrical energy through catalytic reactions of microorganisms under anaerobic conditions. Isolation and characterization of Shewanella sp. from sediments of the Caspian Sea and investigation of its ability to produce electricity were the aims of this study. Samples collected from the sea sediments were cultured in Kligler agar, black colonies were selected. After identification of isolates its ability to produce electricity was evaluated. A two-chamber microbial fuel cell was designed and the selected isolate ME1 was inoculated into the anode chamber containing the LB medium. A sulfate reducing bacterium was isolated from sediments of the Caspian Sea and named as isolate ME1. The results of morphological and physiological characteristics of ME1 showed that this bacterium belonged to the Shewanella sp. Moreover, 16S rRNA sequencing and phylogenetic analyses exhibited that ME1 was similar to Shewanella xiamenensis with 96.38% homology. The results of current studies showed that the isolate ME1 had the ability to produce electricity with a constant open circuit voltage of 765 mV. A maximum power density and a maximum current density were evaluated at 140.817 mW m-2 and 395.50 mA m-2, respectively. The results of this study revealed that the Shewanella sp. ME1 isolated from sediments of the Caspian Sea had the ability to produce electricity in microbial fuel cells. This isolate could be a candidate for the production of electricity in wastewater treatment systems.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
174
187
https://cell.ijbio.ir/article_1112_5f028965221ee05c32939c54e76a0c18.pdf
Recombinant Chitinase produced from a thermophilic Paenibacillus ehimensis
mahdi
mortazavi
shahed university
author
Saeed
Aminzadeh
Academic Staff
author
Alireza
Ghanbari
Shahed University
author
naser
farrokhi
Academic Staff
author
Ali Asghar
Karkhane
Faculty member- National Institute of Genetic Engineering and Biotechnology (NIGEB)
author
zohreh
javaheri
Shahed university
author
text
article
2017
per
Chitin is the second most abundant organic polymer in nature after cellulose and it is the main part of Insects cuticle and crustaceans and includes in cell wall of most fungus and some algae and nematodes. Huge amounts of chitin residual are being produced by organisms that enter to the nature as natural pollutants. However, this polysaccharide has immense application in different aspects of our daily life once becomes digested via chitinases. Chitinase is one of the enzymes that responsible for disintegrating chitin. Some of bacteria produce chitinase for digesting chitin. Here, a thermophilic chitinase gene (JQ675723) obtained from a Paenibacillus ehimensis isolate. The gene was cloned in pET26b and transformed into E. coli to heterologously produce the enzyme. The recombinant protein was isolated via affinity chromatography using Ni-NTA column. The enzyme demonstrated to have hydrolytic activity in the presence of chitin and by addition of 3, 5-dinitrosalicylic acid at 530 nm. Moreover high temperature showed industrial potential. Its nucleotide sequence had high similarity to Glycoside hydrolase family 18. Amino acid sequence from amino to carboxyl determined glycosyl hydrolase domain, fibronectin like domain and chitin binding domain.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
188
197
https://cell.ijbio.ir/article_1089_e13969d21e5b7847c7616921bb9eeca3.pdf
Histopathological evaluation of non-infectious skin deep wound healing activity of Melissa officinalis, Zizyphus spina-christi, Althaea officinalis, Satureja bachtiarica extracts
Mehran
Miroliaei
university of isfahan
author
Reyhaneh
cheloongar
گروه زیست شناسی، دانشکده علوم پایه، دانشگاه پیام نور یزد
author
Akram
Aminjafari
بخش سلولی مولکولی، گروه زیست شناسی، دانشکده علوم، دانشگاه اصفهان
author
اردشیر
طالبی
بخش پاتولوژی، گروه علوم پزشکی، دانشکده پزشکی، دانشگاه اصفهان
author
Majid
Ghias
دانشکده جغرافیا و برنامه ریزی. دانشگاه اصفهان
author
text
article
2017
per
Abstract Despite major advances in treatment of wound, healing remained as one of the most important challenges ahead. Since antioxidant, antimicrobial and anti inflammatory properties of Melissa officinalis, Zizyphus spina-christi, Althaea officinalis, Satureja bachtiarica have been proven and these properties are involved in wound healing, The present investigation was undertaken to assess the efficacy of mentioned officinal herbs hydroalcoholic extracts on thickness and non-septic wound healing model in rat. Each extract was co-administered for 7 and 14 consecutive days on 50 experimental wounded rats. Macroscopic observations showed all applied hydroalcoholic extracts had wound healing potentials. Althaea officinalis extract presented the most activity in wound healing specified by significant increase in wound contraction. Furthermore, histopathological assessments confirmed the wound healing potential of Althaea extract. This could be attributed to the induction effects of a typical fibroblast affecting wound contraction. It was observed a combined hydroalcoholic extract from all herbs also possesses wound healing potential properties less than Althaea officinalis.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
212
222
https://cell.ijbio.ir/article_1118_f6f050fc813f1ffcb551cf7e3cc7e0b8.pdf
Study of propeptide mutation effect on the human factor IX expression
Jafar
Vatandoost
Hakim University
author
Shokoufeh
Hasanabadi
Hakim University
author
Majid
Momeni moghaddam
Hakim University
author
text
article
2017
per
Factor IX as a vitamin K-dependent blood coagulation factor requires post-translational modifications such as gamma carboxylation for its biological function. Gamma carboxylation catalyzed by an enzyme called gamma carboxylase and propeptide of vitamin K dependent proteins is first recognition site of gamma carboxylase. Specific amino acids within these propeptide sequences are responsible for differences in affinity for the gamma carboxylase. Increases of Ki result in decrease of enzyme affinity but fully gamma carboxylation of protein. Since the gamma carboxylase enzyme have least affinity for prothrombin, in this study the pMT-FIX-M13 expression vector constructed contains mutant factor IX cDNA in amino acid -13 (H to P) on the basis of prothrombin propeptide. The expression and activity of the normal and mutant recombinant factor IX were investigated by ELISA and APTT after various times of transfection. The results showed that the concentrations and activity of factor IX and so gamma carboxylation in mutant factor IX is more than healthy factor IX.
Cellular and Molecular Research
(Iranian Journal of Biology)
Iraninan Biology Society
2383-2738
30
v.
2
no.
2017
208
218
https://cell.ijbio.ir/article_1213_35d157a64345e9b8542ca007e97f90fd.pdf